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biolog phenotype microarray (pm) plates  (Biolog Inc)

 
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    Biolog Inc biolog phenotype microarray (pm) plates
    SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
    Biolog Phenotype Microarray (Pm) Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biolog phenotype microarray (pm) plates/product/Biolog Inc
    Average 90 stars, based on 1 article reviews
    biolog phenotype microarray (pm) plates - by Bioz Stars, 2026-03
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    1) Product Images from "Physiological roles of an Acinetobacter -specific σ factor"

    Article Title: Physiological roles of an Acinetobacter -specific σ factor

    Journal: mBio

    doi: 10.1128/mbio.00968-25

    SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.
    Figure Legend Snippet: SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

    Techniques Used: RNA Sequencing, Over Expression, Plasmid Preparation, Control, Knockdown, Two Tailed Test, Activity Assay, Fluorescence, Microarray



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    SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype <t>Microarray</t> PM13.
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    Image Search Results


    SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

    Journal: mBio

    Article Title: Physiological roles of an Acinetobacter -specific σ factor

    doi: 10.1128/mbio.00968-25

    Figure Lengend Snippet: SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

    Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

    Techniques: RNA Sequencing, Over Expression, Plasmid Preparation, Control, Knockdown, Two Tailed Test, Activity Assay, Fluorescence, Microarray

    Data for biolog phenotype microarray PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).

    Journal: Scientific Reports

    Article Title: Characteristics of Corynespora cassiicola , the causal agent of tobacco Corynespora leaf spot, revealed by genomic and metabolic phenomic analysis

    doi: 10.1038/s41598-024-67510-y

    Figure Lengend Snippet: Data for biolog phenotype microarray PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).

    Article Snippet: Figure 3 Data for biolog phenotype microarray PM 1–10 plates of the pathogen Corynespora cassiicola (Utilization of the isolates of C. cassiicola from tobacco was indicated by green areas in the growth curve for each substrate).

    Techniques: Microarray